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specialty journal of biological sciences
Volume 5, 2019, Issue 4
Anti-microbial activities of Raw and Boiled Thevetia peruviana Seed on some ‎selected microorganisms‎
Akintelu Mary Taiwo‎ ‎ ‎
Pages: 49-52


A research work entitled: Anti-microbial activities of raw and boiled Thevetia peruviana ‎seed on some selected microorganisms was carried out in the chemistry laboratory of ‎Federal University of Technology Akure (FUTA), Ondo State, Nigeria. The seed was ‎obtained from Lafe Area of Akure in Ondo State. The study investigated the powdered ‎sample of the raw and boiled seeds of Thevetia peruviana; and the seed extracts were ‎analysed for anti-microbial activities in the treatment of some bacteria diseases. The study ‎also examined the effect of boiling on the sample against the microorganism. Adia et al. ‎‎(2001) method was adopted for the study. The oil from the samples were cultured over each ‎nutrient agar (ASA) plates at 37 0C for 24 hours and were tested against some organisms ‎such as: Escherichia coli, Psuedomonas syringine, Pseudomonas aeruginosa, Bacillus ‎subtilis, Bacillus cereus, Xanthomonas oximopoides and Candida albican. The study ‎revealed that the n-hexane extract exhibited activity against all the organisms tested with ‎zones of inhibition 9-14mm and 0.2-3mm respectively except Escherichia coli, and ‎Pseudomonas syringine that showed no zone of inhibition. The n-hexane extract showed a ‎significantly high activity against Bacillus subtilis and Pseudomonas aeruginosa. Thus, out ‎of the seven selected microorganisms, the raw and boiled seed extract was able to control ‎four of the microbes at 0.5ml concentration used. These microbes are Bacillus subtilis, ‎Bacillus cereus, Pseudomonas aeruginosa, and Xanthomonas. oximopoides. While boiling ‎does not have any effect on the sample against the microorganism at the same concentration ‎used. The study concluded that the presence of anti-microbial activities in the seed revealed ‎high medicinal properties which can be used to treat the infectious diseases created by the ‎tested microorganisms. The study therefore recommends that further studies should be ‎carried out on Thevetia peruviana seed against other microorganism that are not used in this ‎study‎.

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specialty journal of biological sciences
Issue 1, Volume 6, 2020