Pages: 44-53

Abstract

Considering rising antibiotic resistance issues of bacterias, systemic toxicity and drug resistance in chemotherapy, discovering new bio-active compounds is essential. Therefore, introducing new bio-active compounds for treating patients of infectious diseases and cancer can be considered. In this study, the stability of antimicrobial metabolite produced by Pseudomonas sp.UW4 against lipase enzyme and the anti-cancer effect of it was evaluated through the rate of HT29 cell line proliferation and apoptosis. In order to achieve this goal, Pseudomonas sp. UW4 was isolated from Isfahan soil samples and it was evaluated for production of active bio-compounds and stability of antimicrobial metabolite Pseudomonas sp.UW4 against the lipase enzyme. The evaluation of the stability of the produced metabolite against the lipase enzyme showed that this metabolite is persistent against the enzyme. Also, treatment with the metabolite showed that by increasing the concentration of the metabolite depended on dose and time, the biotic potential of the cells decreased. The highest effect was observed at the concentration of 20 mg/ml and 72 hours after treatment of the cells. Induction of apoptosis was also dose-dependent and the concentration of 20 mg / ml supernatant induced the highest percentage of apoptosis in HT29 cells. Therefore, bioactive compounds produced by Pseudomonas sp. UW4 may be used to eliminate infections and treat HT29 breast cancer.