Effects of Crocin and resistance training on liver enzymes and PG axis hormones in toxicated male rats by Nandrolone Decanoate
Hadi LalAbadi, Hassan abbasi, Mohammad Ali Azarbayjani, Maziyar amin
Introduction: The purpose of this study was to investigate the effects of crocin and resistance training on liver enzymes and Hypothalamic - pituitary-gonadal axis hormones in toxicated male rats by Nandrolone decanoate.
Method & materials: Fifty six male Wistar rats (age: 9 weeks, weight: 190±10g) were randomly divided into eight groups: control group (Co, n=7), sham group (Sh), 10 mg/kg of Nandrolone decanoate group (Nd), resistance training group (RT), 12.5 mg/kg of crocin group (C1), 25 mg/kg of crocin group (C2), RT and 12.5 mg/kg of crocin group (RT-C1) , RT and 25 mg/kg of crocin group (RT-C2).All the groups were injected by Nandrolone decanoate (10mlg/kg of body) except (Co and Sh)groups. Resistance training was progressively and consisted of climbing the ladder and started with carrying a load of 50% of body weight. Twenty four hours after the last injection, the animals were sacrificed. Blood samples were obtained from left ventricle immediately and analyzed for liver enzymes (AST, ALT, ALP and GGT), albumin, total protein and PG axis hormones (LH, FSH, testosterone, DHT). Testicular and liver were brought out of their body for analyzing. Data were analyzed using two way analysis of variance.
Results: Data analysis revealed that the interaction effect of crocin and resistance training cause to significant changes on ALT, GGT, Albumin, Total protein, FSH levels(P<0.05). The main effect of crocin on LH, testosterone, DHT, albumin and total protein were significant (P<0.05). Also, there were not any significant changes in number of spermatogonia, primary spermatocytes, spermatids and sertoli cells (P>0.05).
Conclusion: In general, resistance training and crocin consumption result in eliminate or improve of complication of Nandrolone decanoate in liver and testis organ.