Pages: 51-58

Abstract

As different areas of the brain, including the prefrontal area, are involved in the development of ‎addiction, the present study was conducted with the aim of investigating the changes in the expression ‎of mir-382 and FOSBΔ genes in the prefrontal area following alcohol addiction in male Wistar rats. For ‎this purpose, 50 male Wistar rats were prepared from Pharmacology Faculty of University of Tehran and ‎oral alcohol at a dose of 5 mg / kg was administered to animals for 21 days‏. ‏In the next step, using the ‎guillotine device, the head of rats were separated and the considered area was removed. Having used the ‎Real Time PCR (Qigene) method, the expression of FOSBΔ and Mir-382 genes relative to the reference ‎gene was examined. Statistical analysis was performed using SPSS software and Independent T-test. ‎The results showed that following the alcohol addiction for 21 days, the level of expression of Mir382 ‎gene in the prefrontal area decreased, while the expression of the FOSBΔ gene showed an increase. The ‎results of this study showed a reverse relationship between the expression of Mir382 and FOSBΔ genes ‎following alcohol addiction in the prefrontal area of the brain‎‎.