Pages: 32-39

Abstract

The taxonomic position of a novel actinomycete strain, ANTS-1T isolated from soil was clarified using a polyphasic approach. The strain was assigned to the genus Streptomyces based on its phenotypic and cultural characteristics. The isolate produced actinomycin D, an antitumor agent as a principal secondary metabolite. A partial sequence of 16S rDNA of this strain was determined and compared with other Streptomyces species in the NCBI database. Phylogenetic analysis based on partial 16S rDNA sequence indicated that strain ANTS-1T was most closely related to Streptomyces griseoplanus NBRC 12779 (98.93%), Kitasatospora arboriphila HKI 0189 (97.35%) and Streptomyces paucisporeus 1413 (97.35%). However, the phenotypic properties and the nature of secondary metabolite produced by the strain ANTS-1T differed from closely related species of Streptomyces. Thus, the strain ANTS-1T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces banglaensis sp. nov. is proposed.