Pages: 33-39

Abstract

This work incorporates two methods that macroinvertebrate bio-monitoring and ‎DNA barcoding, in an effort to gain a greater understanding of how traditional ‎taxonomic approaches and DNA barcoding, helps in interpretation of water ‎quality and phylogenetic lineage of insects. The present work is carried out to ‎find the effect of the phenomenon in the Kurangani streams from August 2018 to ‎January 2019. Shannon and Simpson index were highest in November 2018 and ‎lowest in January 2019 and it shows climatic changes which have an impact on ‎insect’s diversity. DNA barcoding of Stenopsyche kodaikanelensis which shows ‎close relationship with Chimarra sp and the Indian species S.kodaikanelensis ‎shows closer phylogeny with Chinese species S.brevata. By comparing the ‎barcodes of Trichoptera and Lepidoptera in Kurangani falls, Anisocentropus sp ‎‎(Trichoptera) is in closer lineage with Anthene lycaenina (Lepidoptera) and ‎significantly tells that both arises from a common ancestor‎‎‎.