Investigation of the Changes in the Expression of Mir-382 and FOSBΔ Genes in the Prefrontal Area Following Alcohol Addiction in Male Wistar Rats
Elahe Shahvaroughi, Mostafa Ebadi
As different areas of the brain, including the prefrontal area, are involved in the development of addiction, the present study was conducted with the aim of investigating the changes in the expression of mir-382 and FOSBΔ genes in the prefrontal area following alcohol addiction in male Wistar rats. For this purpose, 50 male Wistar rats were prepared from Pharmacology Faculty of University of Tehran and oral alcohol at a dose of 5 mg / kg was administered to animals for 21 days. In the next step, using the guillotine device, the head of rats were separated and the considered area was removed. Having used the Real Time PCR (Qigene) method, the expression of FOSBΔ and Mir-382 genes relative to the reference gene was examined. Statistical analysis was performed using SPSS software and Independent T-test. The results showed that following the alcohol addiction for 21 days, the level of expression of Mir382 gene in the prefrontal area decreased, while the expression of the FOSBΔ gene showed an increase. The results of this study showed a reverse relationship between the expression of Mir382 and FOSBΔ genes following alcohol addiction in the prefrontal area of the brain.