Phytochemical and Antibacterial Potential of Punica granatum L. Leaves, Flower and Stem Bark Extracts against Human Pathogenic Bacteria
Javid Ali, Shamsur Rehman, Jehangir Shah, Muhammad Siddique Afridi
The phytochemical and antibacterial activities’ study of the Punica granatum leaves, flower and stem bark extracts were carried out. The phytochemical content was carried out according to the standard operated procedures. The antibacterial activities were carried out by standard agar well diffusion method. The methanol extract showed the highest presence of phytochemical, followed by ethanol, water and acetone extract. While, leaves showed the highest presence of phytochemical as compared to flower and stem bark. The methanol leaves’ extract showed maximum zone of inhibition 21±01mm and 20±01 mm against Enterococcus fecalis and Staphylococcus aureus respectively. The aqueous leaves extract showed 19±0.5 mm zone of inhibition against Enterococcus fecalis. Ethanol leaves’ extract observed 20±01 mm zone of inhibition against Escherichia coli. Generally, acetone leaves were found least zone of inhibition 09±0.4 mm against Pseudomonas aeruginosa. Maximum zone of inhibition 20±0.1 was noted against Escherichia coli by methanol flower extract and lowest zone of inhibition 07±0.4 mm was found by acetone flower extract against Pseudomonas aeruginosa. While stem bark acetone extract showed a low zone of inhibition 02±00 mm against Enterococcus fecalis and maximum zone of inhibition 15±0.2 mm by methanol extract against Salmonella typhi in this regard. These findings propose that pomegranate parts’ extract has a considerable capacity to prepare a new broad spectrum of antibiotic plant based formulations.