The Effect of Anaerobic Exercise with Melatonin Consumption on HSP60 and HSP70 in Rat Myocard After Ischemic-Reperfusion
Masoud Jokar, Kambiz Moradi Dehbaghi, Sadegh Azizkhani, Hamid Rajabi, Pezhman Motamedi
Introduction: Physical activity and foods containing antioxidants seem to be beneficial for cardiovascular disease prevention. The present research aims to examine the effect of anaerobic exercise with melatonin consumption on the expression of HSP60 and HSP70 markers in rat myocard after ischemic-reperfusion. Methods: 30 male Wistar rats weighted approximately 200-250 g and aged two to three months old were used. The pilot group (N = 14) was divided into healthy group without isoprenaline induction (N=7) and the isoprenaline-induced group with (N = 7). In following, rats in melatonin group (N = 4) were gavaged every day for one month using a dose of 10 mg/kg BW. Meanwhile, rats in anaerobic group and melatonin anaerobic group were exposed tredmill training course with frequency of three times per week for one month. Finally, rats were sacrificed after confirmation of infarct and expression of HSP60 and HSP70 gene were studied by real-time method. Results: There was a significant difference between the experimental groups in terms of changes in HSP70 genes expression. It was found that there was no significant difference between the two anaerobic and melatonin anaerobic groups (sig.=0.989). There was no significant difference between the experimental groups in terms of changes in HSP70 genes expression because the significance was estimated 0.297 which was greater than 0.05. Conclusion: Melatonin therapy and anaerobic training have negligible effect on HSP60 and HSP70 gene expression. But, anaerobic exercise with consuming melatonin can decrease and increase HSP60 and HSP70 gene expression respectively but don’t show a significant effect compared to melatonin therapy alone.